LOCUS       40924_48818        3896 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector Sos_ZsG_pol I-mg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, 
            Nichol ST, Spiropoulou CF, Albarino CG.
  TITLE     Development of a reverse genetics system for Sosuga virus allows 
            rapid screening of antiviral compounds
  JOURNAL   PLoS Negl Trop Dis 12 (3), e0006326 (2018)
  PUBMED    29522528
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Albarino C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 
            30329, USA
REFERENCE   3  (bases 1 to 3896)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis"; date: "2018"; volume: "12"; issue: "3"; pages: "e0006326"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Viral Special Pathogens Branch, Centers for Disease 
            Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..35
                     /label=pol I terminator
                     /note="pol I terminator"
                     /regulatory_class="terminator"
     CDS             200..892
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     regulatory      complement(962..1173)
                     /label=pol I promoter
                     /note="pol I promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1268..1284)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1292..1322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1337..1358)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2408..3265)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3266..3370)
                     /label=AmpR promoter
     primer_bind     3844..3860
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accggagtac tggtcgacct ccgaagttgg gggggaccag agggaaaatt cagaagggtc
       61 gttctcgata gaggaaggtc ctgatattgt gagcccggaa gtggcaaggc tcggagtcga
      121 aatcgacttt gagcttgttc ttccggagcc tctgacttgc ctgatttcat aagtcagcct
      181 ttctctgttg ttggaagcca tggcccagtc caagcacggc ctgaccaagg agatgaccat
      241 gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg gcgagggcat
      301 cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg gcggcccctt
      361 gcccttcgcc gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg tgttcaccga
      421 gtacccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct acacctggga
      481 ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca tcaccgtgag
      541 cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact tccccgccga
      601 cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga agatcatccc
      661 cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg
      721 tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc cccgcaagat
      781 gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg acgccaagaa
      841 ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc cctgattgac
      901 taggaagaga aaatgcagtc tcagatattt aagaaaaaac ttatagattt tccccttggt
      961 caataacccg gcggcccaaa atgccgactc ggagcgaaag atatacctcc cccggggccg
     1021 ggaggtcgcg tcaccgacca cgccgccggc ccaggcgacg cgcgacacgg acacctgtcc
     1081 ccaaaaacgc caccatcgca gccacacacg gagcgcccgg ggccctctgg tcaaccccag
     1141 gacacacgcg ggagcagcgc cgggccgggg acgccctccc ggcggtatcg gatcccgggc
     1201 ccgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1321 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1381 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3361 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3421 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3481 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3541 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3601 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     3661 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     3721 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     3781 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3841 gttgtaaaac gacggccagt gaattcgagc tcggtacctc gcgaatgcat ctagat
//
